Several meristem culture media have been reported in the literature. The meristem culture medium used at the ICAR-CPRI is based on MS basal nutrients supplemented with 2mg/l D-calcium pantothenate, 0.1 mg/l GA3 and 30 g/l sucrose, and solidified with 7.0 g/l agar. For chemotherapy, meristem culture medium is also supplemented with suitable concentration of anti-viral compounds.

It takes about 5-6 months for meristems to grow into full plantlets (mericlones). At this stage sub-culture the plantlets individually for maintaining their clonal identity.