Potato Aucuba Mosaic Virus
Potato Aucuba Mosaic Virus (PAMV) is an uncommon pathogen of potato (Solanum
tuberosum) which occurs worldwide. It is a single stranded RNA (ssRNA) virus with
filamentous particles about 580 nm long and 11 nm wide and belongs to genus Potexvirus;
family Alphaflexiviridae; order Tymovirales. PAMV is transmitted by aphids (Myzus
persicae) in the presence of ‘helper’ viruses of the PVY group in a non-persistent manner and
is readily transmitted by mechanical inoculation. It causes bright yellow spots on the lower
leaves, later coalescing to form large yellow necrotic spots, often leading to systemic or top
necrosis. Particles of potexviruses are composed of a single type of coat protein, which
encapsidates a single molecule of 5' capped and 3' polyadenylated positive-stranded RNA
differing in length from about 5.8 to 7 kb. The genomic RNA of PAMV is 7057 nucleotides
in length excluding the poly (A) tail at the 3' end. The non-coding regions are 82 nucleotides
at the 5' end and 76 nucleotides at the 3' end. A putative polyadenylation signal (AAUAAA)
is also found at the extreme 3' end of the RNA and includes a part of the poly (A) tail. The
genome contains five major putative open reading frames (ORFs), designated from the 5'
terminus as encoding putative proteins with M of 187 K, 26 K, 12 K, 8 K and 27 K. The sizes
and composition of the proteins encoded by the ORFs are generally similar to those found in
other potexviruses.